Augmentation of demineralized bone matrix (DBM) mineralization by a synthetic growth factor mimetic

✍ Scribed by Xinhua Lin; Louis A. Peña; Paul O. Zamora; Sarah L. Campion; Kazuyuki Takahashi

• Publisher: Elsevier Science
• Year: 2006
• Tongue: English
• Weight: 360 KB
• Volume: 24
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.20215

✦ Synopsis

Abstract

These studies evaluated whether F2A4‐K‐NS, a peptide mimetic of FGF‐2, could augment ectopic bone production following the subcutaneous implant of human demineralized bone matrix (DBM). DBM was formulated into a gel with and without F2A4‐K‐NS, and injected subcutaneously into athymic rats. After 28 days the resultant tissue was excised and fixed. The tissue was examined with soft X‐rays and microcomputerized tomography (micro‐CT), and by histological methods. Inclusion of F2A4‐K‐NS with DBM resulted in an increased mineral deposition as determined by soft X‐ray and micro‐CT analysis and von Kossa staining. DBM‐containing tissues showed extensive mineralization compared to the carrier alone, which was poorly mineralized. The mineralization was qualitatively and quantitatively the most extensive in the samples containing F2A4‐K‐NS plus DBM. Additionally, the highest amount of von Kossa staining for calcium was observed in tissues from animals that had received DBM plus F2A4‐K‐NS. In these studies, 100 ng of peptide per 0.2 mL of injectable DBM gel generated the most optimal results. The synthetic peptide F2A4‐K‐NS augmented DBM‐induced ectopic mineralization in athymic animals. © 2006 Orthopaedic Research Society. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:2051–2058, 2006