Attempts to produce in vitro the reaction of immunologically competent cells of C57BL/6 origin to Balb/c embryo fibroblasts

✍ Scribed by P. Koldovský; A. Turano; G. Fadda

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 549 KB
• Volume: 74
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040740105

✦ Synopsis

Spleen or lymph node cells of C57BL/6 origin or both were mixed with subcellular material from Balb/c tissue for several hours at 37°C. The subcellular material was then washed out (by repeated centrifugation) with PBS and the immune cells were placed either in diffusion chambers in the peritoneal cavity of Swiss mice or in Petri dishes with C57BL/6 embryo cell monolayer (feeder layer) and incubated in a thermostat at an atmosphere of 5% COZ. After 4 to 5 days these cells were added to target Balb/c embryo cells in Petri dishes. The first control was done by sensitizing the cells with syngeneic material, the second control by adding the immune cells to syngeneic target embryo cells. The sensitized cells mixed with allogeneic embryo cells produced a positive reaction in the form of agglutination around the target cells and destruction of these cells. In controls the immune cells were seldom found and were randomly distributed.