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Attachment of labeled TMV to tobacco mesophyll protoplasts

✍ Scribed by Yu.N. Zhuravlev; N.F. Pisetskaya; L.A. Shumilova; T.I. Musorok; V.G. Reifman


Book ID
119008980
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
495 KB
Volume
64
Category
Article
ISSN
0042-6822

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Plating of isolated tobacco mesophyll pr
✍ Toshiyuki Nagata; Itaru Takebe πŸ“‚ Article πŸ“… 1971 πŸ› Springer-Verlag 🌐 English βš– 718 KB

A technique was developed to derive cell and plant clones from isolated mesophyll protoplasts of tobacco. The protoplasts, plated on a fully defined agar medium, divided and grew actively forming visible colonies after one month of culture. Efficiency of colony formation depended on cell density and