𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Attachment of fluorescent dyes to polyacrylamides in aqueous media

✍ Scribed by Robert Borg; Mitchell A. Winnik

Book ID
105337558
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
476 KB
Volume
28
Category
Article
ISSN
0887-624X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Fluorescent derivatives of polyacrylamide can be prepared by reacting an acidic aqueous solution of the polymer with xanthenol and dibenzosuberenol. These reactions take advantage of the carbocation stability of these dyes when they are treated with acid (i.e., pK~R+~ values near zero). In this way the O‐alkylated amide that one expects to be formed is too unstable to persist, and, eventually, the stable N‐alkylated derivative is formed. UV absorption and fluorescence spectra for the labelled polymers are reported. The presence of the dye affects the hydrophobic–hydrophilic balance of the polymer.

📜 SIMILAR VOLUMES

A Core–Shell Nanoparticle Approach to Ph
A Core–Shell Nanoparticle Approach to Photoreversible Fluorescence Modulation of a Hydrophobic Dye in Aqueous Media
✍ Jian Chen; Fang Zeng; Shuizhu Wu; Qiming Chen; Zhen Tong 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 405 KB

## Abstract Amphiphilic core–shell nanoparticles containing spiropyran moieties have been prepared in aqueous media. The nanoparticles consist of hydrophilic and biocompatible poly(ethyleneimine) (PEI) chain segments, which serve as the shell, and a hydrophobic copolymer of methyl methacrylate (MMA

Fluorescent dyes bound to hydrophilic co
Fluorescent dyes bound to hydrophilic copolymers: Applications in aqueous halide sensing
✍ Chris D. Geddes; Peter Douglas 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 285 KB

A range of halide-sensitive fluorophores were bound to two hydrophilic copolymers. Thin films of the copolymers swelled in the aqueous media, allowing dye fluorescence to be dynamically quenched by the diffusion of halide ions. The resultant sensor films were characterized in terms of their hydrophi

Physical attachment of fluorescent prote
Physical attachment of fluorescent protein particles to atomic force microscopy probes in aqueous media: Implications for surface pH, fluorescence, and mechanical properties studies
✍ Susana Moreno-Flores; Radostina Georgieva; Yu Xiong; Kathryn Melzak; Hans Bäumle 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 331 KB 👁 1 views

## Abstract Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so‐attached particle can in turn act as a probing tool to study particle–substrate and particle–particle interactions. Attach