Atrial cardiomyocyte-specific expression of Cre recombinase driven by an Nppa gene fragment

✍ Scribed by Frederik J. de Lange; Antoon F.M. Moorman; Vincent M. Christoffels

• Book ID: 102222818
• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 246 KB
• Volume: 37
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/gene.10220

✦ Synopsis

Abstract

Summary: To study the development of the atria, we produced a transgenic mouse line that expresses Cre under the regulatory control of a 7 kbp fragment of the Natriuretic peptide precursor type A gene (Nppa), from −3 kbp to +4 kbp relative to the transcription start site. Crossing this line with the R26R and Z/EG reporter lines revealed recombinase activity specifically in the cardiomyocytes of the atria and to a lesser extent the inflow tract from E10.5 onwards. At E14.5 recombination in the atria is almost complete. No recombination was observed outside the heart. These mice provide a tool to study gene function in the atria. genesis 37:1–4, 2003. © 2003 Wiley‐Liss, Inc.