Atomic Force Microscopy of DNA Molecules Stretched by Spin-Coating Technique

✍ Scribed by Jing Yong Ye; Kazuo Umemura; Mitsuru Ishikawa; Reiko Kuroda

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 370 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4544

✦ Synopsis

We have developed an effective approach to stretching DNA molecules with the flow of fluid generated by spin coating. Well-stretched DNA molecules were observed using atomic force microscopy. Substrate properties sensitively affected the stretching behavior of DNA. Our experimental findings revealed that a mica surface treated with crystal violet, a cationic dye molecule, is suitable to the spin-coating procedure for stretching DNA. Moreover, compared with relaxed DNA, we observed reduced height of the stretched DNA, which was attributed mainly to elongation force applied to the DNA molecules from the fluid flow and strong adhesion force between DNA and the substrate. This simple and effective method for preparing stretched DNA could be useful in physically mapping genomic DNA in a high throughput.