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Atomic Force Microscopy: Methods and Protocols

✍ Scribed by Nuno C. Santos, Filomena A. Carvalho

Publisher
Springer New York,Humana Press
Year
2019
Tongue
English
Leaves
369
Series
Methods in Molecular Biology 1886
Edition
1st ed.
Category
Library
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✦ Synopsis

This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Artifacts and Practical Issues in Atomic Force Microscopy (Peter Eaton, Krystallenia Batziou)....Pages 3-28
Quantitative Analysis of Structure and Dynamics in AFM Images of Lipid Membranes (Simon D. Connell, George R. Heath, James A. Goodchild)....Pages 29-44
Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy (Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, Cédric Godefroy et al.)....Pages 45-59
Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscopy (Subhadip Senapati, Paul S.-H. Park)....Pages 61-74
Analysis of DNA–Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2–Telomeric DNA Wrapping (Sabrina Pisano, Eric Gilson)....Pages 75-97
Cell Topography and Its Quantitative Imaging by AFM (Jiang Pi, Jiye Cai)....Pages 99-113
Front Matter ....Pages 115-115
Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognition Imaging (A. Ebner, L. Wildling, H. J. Gruber)....Pages 117-151
Ligand-Receptor Binding on Cell Membrane: Dynamic Force Spectroscopy Applications (Jianli Liu, Wenhui Li, Xuejie Zhang, Yan Feng, Xiaohong Fang)....Pages 153-162
Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations (Fidan Sumbul, Felix Rico)....Pages 163-189
AFM to Study Pore-Forming Proteins (Joseph D. Unsay, Ana J. García-Sáez)....Pages 191-202
Imaging and Manipulation of Extracellular Traps by Atomic Force Microscopy (Ricardo H. Pires, Mihaela Delcea, Stephan B. Felix)....Pages 203-217
Front Matter ....Pages 219-219
Investigation of Bacterial Curli Production and Adhesion Using AFM (Yoo Jin Oh, Peter Hinterdorfer)....Pages 221-231
Antimicrobial Peptides: Effect on Bacterial Cells (Marco M. Domingues, Mário R. Felício, Sónia Gonçalves)....Pages 233-242
AFM Nanoindentation Experiments on Protein Shells: A Protocol (Yukun Guo, Wouter H. Roos)....Pages 243-257
Structural and Mechanical Characterization of Viruses with AFM (Álvaro Ortega-Esteban, Natália Martín-González, Francisco Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Cármen San MartÚn et al.)....Pages 259-278
Front Matter ....Pages 279-279
Quantification of the Elastic Properties of Soft and Sticky Materials Using AFM (Nicolas Bouchonville, Alice Nicolas)....Pages 281-290
Measuring the Elastic Properties of Living Cells (Hermann Schillers)....Pages 291-313
Measuring Elastic Properties of Single Cancer Cells by AFM (Małgorzata Lekka, Joanna Pabijan)....Pages 315-324
Front Matter ....Pages 325-325
Molecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitro (Carla P. Gomes, Hugo Oliveira, Andreas Ebner, Peter Hinterdorfer, Ana P. Pêgo)....Pages 327-341
Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy (Jan Pribyl, Martin Pešl, Guido Caluori, Ivana Acimovic, Sarka Jelinkova, Petr Dvorak et al.)....Pages 343-353
Simultaneous AFM Investigation of the Single Cardiomyocyte Electro-Chemo-Mechanics During Excitation-Contraction Coupling (Guido Caluori, Roberto Raiteri, Mariateresa Tedesco)....Pages 355-367
Back Matter ....Pages 369-372

✦ Subjects

Life Sciences; Biological Microscopy

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