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Atomic force microscopy: Bombyx mori silk fibroin molecules and their higher order structure

✍ Scribed by Shun-Ichi Inoue; Jun Magoshi; Toshihisa Tanaka; Yoshiko Magoshi; Mary Becker


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
295 KB
Volume
38
Category
Article
ISSN
0887-6266

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✦ Synopsis


We performed atomic force microscopy measurements on fibroin molecules from the domestic silkworm, Bombyx mori. At low concentrations, we could observe single protein molecules. The shape of the observed molecules is a rod with long smaller chains extending from the ends. The size of the rod is 60 nm in length and 15 nm in width. At high concentrations, we observed long threadlike aggregates of fibroin molecules, their chains entangled with one another. These results provide insight into the microscopic mechanism of silk-fiber formation.