✦ LIBER ✦

Atom force microscopic characterisation of the interaction forces between bovine serum albumin and cross-linked alkylated chitosan membranes in media of different pH

✍ Scribed by Wen Guang Liu; Fang Li; Xiao Dong Zhao; Kang De Yao; Qing Gang Liu

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 91 KB
Volume: 51
Category: Article
ISSN: 0959-8103
DOI: 10.1002/pi.1085

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✦ Synopsis

Abstract

Butyl, octyl and hexadecyl moieties were introduced into chitosan. The adhesion of bovine serum albumin (BSA) onto glucose aldehyde‐crosslinked alkylated chitosan membranes in pH media was investigated by probing the force‐displacement curves with BSA‐coated Atom force microscope (AFM) tips. The results indicated that, at the isoelectric point (IP), the sample membranes exhibited higher adhesion forces; and deviating from IP ie at pH 2, pH 6, the adhesion forces decreased. The adhesion forces at pH 2 are less than those at pH 6 due to the presence of electrostatic repulsive and attractive interactions, respectively. Measurements of the adhesion force confirmed quantitatively that the introduction of hydrophobic side‐chains to chitosan can facilitate protein adsorption; however, longer flexible side‐chains can depress protein adsorption to a certain degree. From an analyses of the adhesion forces, it is proposed that protein adsorption can be tuned by adjusting the lengths of the introduced side‐alkyl moieties.