ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation

Complex H2AX phosphorylation patterns by

Complex H2AX phosphorylation patterns by multiple kinases including ATM and DNA-PK in human cells exposed to ionizing radiation and treated with kinase inhibitors

✍ Hongyan Wang; Minli Wang; Huichen Wang; Wilfried Böcker; George Iliakis 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 464 KB

## Abstract In eukaryotic cells, DNA double strand breaks (DSBs) cause the prompt phosphorylation of serine 139 at the carboxy terminus of histone H2AX to generate γ‐H2AX, detectable by Western blotting or immunofluorescence. The consensus sequence at the phosphorylation site implicates the phospha