Asymmetrically functional surface properties on biocompatible phospholipid polymer membrane for bioartificial kidney
✍ Scribed by Hideto Ueda; Junji Watanabe; Tomohiro Konno; Madoka Takai; Akira Saito; Kazuhiko Ishihara
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 391 KB
- Volume
- 77A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
To obtain a bioartificial kidney composed of a porous polymer membrane and renal cells, a polysulfone (PSf) membrane (PSM) blended with 2‐methacryloyloxyethyl phosphorylcholine (MPC) polymer was prepared. The PSM flat membrane with a porous structure could be prepared from the polymer blend containing 1 wt % of the MPC polymer in PSf by the phase inversion technique in a dry–wet process. Asymmetrical surface properties were observed on both sides of the membrane surfaces. That is, the sponge layer formed at the substrate‐contacting surface of the membrane had 10–20 μm pores, but the pores in the micrometer range could not be observed for a skin layer formed at the air‐contacting surface of the membrane. At the sponge layer surface, the MPC unit composition was 7 times larger than that at the skin layer surface. The amount of proteins adsorbed on the surface corresponded to the MPC unit composition. On the skin layer, a small amount of adsorbed proteins and platelet adhesion could be suppressed compared with those on the sponge layer. However, the skin layer had a moderate protein adsorption, so it showed a sufficient cytocompatibility to enable renal tubule epithelial cells to adhere and proliferate in the membrane. Thus, it functioned well as a renal tubule. Therefore, because of both its hemocompatibility and cytocompatibility, we could conclude that the PSM membrane is useful for as a renal tubule device for a bioartificial kidney. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006