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Asymmetric distribution of phospholipids in membranes from Mycobacterium phlei

✍ Scribed by Gyanendra Kumar; Vijay K. Kalra; Arnold F. Brodie

Book ID: 115704113
Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 934 KB
Volume: 198
Category: Article
ISSN: 0003-9861
DOI: 10.1016/0003-9861(79)90391-6

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## Abstract The membrane bound coupling factor‐latent ATPase was solubilized from the membrane vesicles of Mycobacterium phlei by using 0.25 M sucrose or low ionic strength buffer. Purification of the solubilized enzyme by use of Sepharose‐ADP conjugate gel yielded a homogenous preparation of laten