Asymmetric distribution of phosphatidylethanolamine inC. albicans : possible mediation byCDR1, a multidrug transporter belonging to ATP binding cassette (ABC) superfamily
✍ Scribed by Dogra, Smriti; Krishnamurthy, Shankarling; Gupta, Vinita; Dixit, Bharat L.; Gupta, C. M.; Sanglard, Dominique; Pprasad, Rajendra
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 263 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans, is present in its external half. Evidence is presented to show that the availability of PtdEtn could be related to the expression of a multidrug transporter CDR1 of C. albicans, and the process is energy-dependent. A homozygous CDR1 disruptant strain of C. albicans shows almost 23% reduction in the external labelling of PtdEtn. This report shows that, similar to human MDRs, yeast multidrug transporter could also be involved in aminophospholipid translocation.