Asymmetric Binding of the Inhibitor di(adenosine-5′) Pentaphosphate (Ap5A) to Adenylate Kinase
✍ Scribed by B. D. Nageswara Rao and Mildred Cohn
- Book ID
- 123654603
- Publisher
- National Academy of Sciences
- Year
- 1977
- Tongue
- English
- Weight
- 632 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/67353
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📜 SIMILAR VOLUMES
A biochemical assay for the measurement of ATP synthesis coupled to electron transport in the presence of adenylate kinase was developed as an alternative to using the conventional Clark-type oxygen electrode. The assay utilizes Pi,p-di-(adenosine-5')-pentaphosphate which is shown to he a competitiv
P1,P5-di(adenosine 5')pentaphosphate (Ap5A) is an excellent inhibitor of human hemolysate adenylate kinase at concentrations near 2 microM and above. At ten times this concentration and in hemolysate enzyme assays under conditions described in this paper it appears not to alter reaction data in the