Astrocytes: Methods and Protocols (Methods in Molecular Biology, v814)

✍ Scribed by Richard Milner

Publisher: Springer
Year: 2011
Tongue: English
Leaves: 590
Edition: 2012
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS, including generation of neural stem cells, synaptogenesis, and regulation of the blood-brain barrier and neurovascular unit. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

📜 SIMILAR VOLUMES

Molecular Chaperones: Methods and Protoc

📁 Molecular Chaperones: Methods and Protocols (Methods in Molecular Biology, v787)

✍ Stuart K. Calderwood, Thomas L. Prince 📂 Library 📅 2011 🏛 Humana Press 🌐 English

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecula

SELDI-TOF Mass Spectrometry: Methods and

📁 SELDI-TOF Mass Spectrometry: Methods and Protocols (Methods in Molecular Biology, v818)

✍ Charlotte H. Clarke, Diane L. Bankert McCarthy 📂 Library 📅 2011 🏛 Springer 🌐 English

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characte

Biological Aging: Methods and Protocols

📁 Biological Aging: Methods and Protocols (Methods in Molecular Biology Vol 371)

✍ Trygve O. Tollefsbol 📂 Library 📅 2007 🌐 English

This book investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedure

Molecularly Imprinted Polymers: Methods

📁 Molecularly Imprinted Polymers: Methods and Protocols (Methods in Molecular Biology, 2359)

✍ Antonio Martín-Esteban (editor) 📂 Library 📅 2021 🏛 Humana 🌐 English

<span>This detailed volume explores molecularly imprinted polymers (MIPs), which have attracted great interest both in fundamental research and for practical applications due to their selective molecular recognition capabilities, extraordinary stability, and ease of preparation. Beginning with key l

Myogenesis: Methods and Protocols (Metho

📁 Myogenesis: Methods and Protocols (Methods in Molecular Biology, v798)

✍ Joseph X. DiMario 📂 Library 📅 2011 🏛 Springer 🌐 English

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for rese

mTOR: Methods and Protocols (Methods in

📁 mTOR: Methods and Protocols (Methods in Molecular Biology, v821)

✍ Thomas Weichhart 📂 Library 📅 2011 🏛 Springer 🌐 English

Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many