## Abstract We have used the polymerase chain reaction and dideoxynucleotide sequencing to amplify and sequence exons 1 and 2 of the cโKiโ__ras__ protoโoncogene from the normal Syrian golden hamster. Similar methods were employed to screen for the presence of point mutations in the cโKiโ__ras__ onc
Association of point mutation in c-Ki-ras oncogene in lung adenocarcinoma with particular reference to cytologic subtypes
โ Scribed by Toshiaki Kobayashi; Hitoshi Tsuda; Masayuki Noguchi; Setsuo Hirohashi; Yukio Shimosato; Tomoyuki Goya; Yoshihiro Hayata
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 667 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0008-543X
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## Abstract We investigated point mutational activation of the __ras__ genes (Kโ__ras__ codons 12, 13 and 61; Nโ__ras__ codons 12, 13 and 61; Hโ__ras__ codons 12 and 61) in primary, resected lung cancer by dot blotting and oligonucleotide hybridization. Kโ__ras__ mutations were found in 14 (29%) of
## Abstract To study the possible role of __ras__ oncogene activation in the dissemination of colon cancer, we determined point mutations in codons 12, 13 and 61 in Kโ and Nโ__ras__ in 3 groups of tumors: (A) primary tumors of patients who had undergone surgery for Dukes' B (earlyโstage) colon canc