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Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome

โœ Scribed by Grainger, D. C.; Hurd, D.; Goldberg, M. D.; Busby, S. J. W.

Book ID
115536331
Publisher
Oxford University Press
Year
2006
Tongue
English
Weight
920 KB
Volume
34
Category
Article
ISSN
0305-1048

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The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).