Association of neuronal pp60c-src with growth cone glycoproteins of rat brain

✍ Scribed by Hanissian, Silva H. ;Chatila, Talal ;Sahyoun, Naji E.

• Book ID: 102958377
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 962 KB
• Volume: 23
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/neu.480230703

✦ Synopsis

Tyrosine phosphorylation and protein tyrosine kinase (F'TK) activity in the growth cone membrane-associated glycoprotein (GCGP) fraction of 1-day-old rat brain were examined. Using immunohlotting and immunoprecipitation techniques, pp60""" was identified as one of the major PTKs associated with GCGPs. Furthermore, only GCGP-associated src that was also tyrosine phosphorylated was active. Immunoprecipitation experiments using various src antibodies revealed that pp60c-s'c contributed partially to the PTK activity detected in GCGPs, and that it is associated with several proteins of Mr 140 K, 120 K, 85 K and 50 K. This association of src protein with GCGPs was specific, and another src family