Association of neuronal nitric oxide synthase (nNOS) with α1-syntrophin at the sarcolemma

✍ Scribed by Yuko Miyagoe-Suzuki; Shin'Ichi Takeda

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 112 KB
• Volume: 55
• Category: Article
• ISSN: 1059-910X
• DOI: 10.1002/jemt.1167

✦ Synopsis

Abstract

α1‐syntrophin is a PDZ‐containing dystrophin‐associated protein, expressed predominantly in striated muscle and brain. α1‐syntrophin null mice generated by gene targeting technique showed no overt muscular dystrophic phenotype. Though other dystrophin‐associated proteins were localized at the sarcolemma, neuronal nitric oxide synthase (nNOS) was selectively lost from the membrane fraction but remained in the cytoplasm. Thus, the α1‐syntrophin null mice are useful in the elucidation of the functional importance of nNOS targeting at the sarcolemma. In addition, the mice would facilitate identification of other signaling molecules, which are targeted to dystrophin complex via interaction with α1‐syntrophin. Microsc. Res. Tech. 55:164–170, 2001. © 2001 Wiley‐Liss, Inc.