Association of different tumor necrosis factor α promoter allele frequencies with ankylosing spondylitis in HLA-B27 positive individuals
✍ Scribed by Thomas Höhler; Thomas Schäper; Peter M. Schneider; Karl-H. Meyer zum Büschenfelde; Elisabeth Märker-Hermann
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 467 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. To investigate the potential association of tumor necrosis factor a (TNFa) promoter alleles with ankylosing spondylitis.
Methods. DNA from 141 HLA-B27 positive Caucasian patients with ankylosing spondylitis and 46 B27-positive and 99 B27-negative healthy Caucasian controls was investigated by polymerase chain reaction amplification of the TNFa promoter region and subsequent dot-blot analysis with allele-specific oligonucleotides.
Results. There was a significant decrease in the promoter alleles TNF-238.2 and TNF-308.2 in the ankylosing spondylitis group (266 wild-type alleles, 16 variant alleles) compared with the B27-positive (75 wildtype promoter alleles, 17 variant alleles; P < 0.0004) and the B27-negative (159 wild-type promoter alleles, 39 variant alleles; P < 0.00001) control groups. Positivity for the variant promoter alleles conferred protection and a relative risk of 0.3 to B27-positive individuals.
Conclusion. These data indicate that allelic variations in the TNFa promoter influence disease susceptibility in HLA-B27 positive individuals. This protective effect of variant promoter alleles could be related to differences in TNFa production or could reflect the association of different B27 haplotypes with ankylosing spondylitis.