Association of a GDP binding activity with initiation factor eIF-2 from calf liver
โ Scribed by Evan A. Stringer; Asok Chaudhuri; Umadas Maitra
- Book ID
- 118311624
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 395 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0006-291X
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Cell-free translation systems prepared from suspension-cultured HeLa S3 cells or mouse I cells by hypotonic shock followed by Dounce homogenization poorly initiated the translation of exogenous mRNA. In contrast, cell extracts prepared from cells exposed to the detergent lysolecithin translated exog
A cap binding complex activity was purified from HeLa cells by a procedure which does not depend on the use of cap-affinity chromatography. The activity co-purified with a Mr 220,000 polypeptide (p220), but not with eIF4A. The active complex therefore differs from eIF4F, the complex purified by cap