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Association-dissociation of purified subunits of GTP-binding proteins measured by fluorescence energy transfer

✍ Scribed by Marga Koestler; Helmut Heithier; Martin Baumann; Christian Dees; Mirko Hekman; Winchil Vaz


Book ID
112587716
Publisher
Springer
Year
1989
Tongue
English
Weight
67 KB
Volume
8
Category
Article
ISSN
1573-4943

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Fluorescence energy transfer measurement
✍ Anusree Bhattacharya; Siddhartha Roy; Bhabatarak Bhattacharyya πŸ“‚ Article πŸ“… 1996 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 754 KB

## Abstract 9‐(Dicyanovinyl) julolidine (DCVJ) is a fluorescent probe, which binds to a unique site on the tubulin dimer and exhibits different properties that are dependent upon its oligomeric state (Kung & Reed, 1989). DCVJ binds to tubulin, the tubulin‐colchicine complex, and the tubulin‐rutheni