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Assignments, secondary structure and dynamics of the inhibitor-free catalytic fragment of human fibroblast collagenase

โœ Scribed by Franklin J. Moy; Michael R. Pisano; Pranab K. Chanda; Charlotte Urbano; Loran M. Killar; Mei-Li Sung; Robert Powers


Book ID
110369496
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
343 KB
Volume
10
Category
Article
ISSN
0925-2738

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## Abstract A recombinant 19โ€kDa human fibroblast collagenase catalytic fragment modeled on a naturally occurring proteolytic product was purified from __E. coli__ inclusion bodies. Following renaturation in the presence of zinc and calcium, the fragment demonstrated catalytic activity with the sam