𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Assignment of the 13C-nmr spectra of virgin and reactive-site modified turkey ovomucoid third domain

✍ Scribed by A. D. Robertson; G. I. Rhyu; W. M. Westler; J. L. Markley

Book ID
101719722
Publisher
Wiley (John Wiley & Sons)
Year
1990
Tongue
English
Weight
455 KB
Volume
29
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

The virgin (reactive-site Leu'8-Glu'9 peptide bond intact) and modified (reactive-site Leu'X-Glu'9 peptide bond hydrolyzed) forms of turkey ovomucoid third domain (OMTKY3 and OMTKY3*, respectively) have been analyzed by proton-detected 'H{I3C} twodimensional single-bond correlation (1H{13C}SBC) spectroscopy. Previous 'H-nmr assignments of these proteins [A

πŸ“œ SIMILAR VOLUMES

NMR chemical shift mapping of the bindin
NMR chemical shift mapping of the binding site of a protein proteinase inhibitor: changes in the 1H, 13C and 15N NMR chemical shifts of turkey ovomucoid third domain upon binding to bovine chymotrypsin AΞ±
✍ Jikui Song; John L. Markley πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 201 KB

## Abstract The substrate‐like inhibition of serine proteinases by avian ovomucoid domains has provided an excellent model for protein inhibitor‐proteinase interactions of the standard type. ^1^H,^15^N and ^13^C NMR studies have been undertaken on complexes formed between turkey ovomucoid third dom

Increasing the hydrolysis constant of th
Increasing the hydrolysis constant of the reactive site upon introduction of an engineered Cys14Cys39 bond into the ovomucoid third domain from silver pheasant
✍ Hikaru Hemmi; Takashi Kumazaki; Shuichi Kojima; Takuya Yoshida; Tadayasu Ohkubo; πŸ“‚ Article πŸ“… 2011 πŸ› John Wiley and Sons 🌐 English βš– 203 KB

## Abstract P14C/N39C is the disulfide variant of the ovomucoid third domain from silver pheasant (OMSVP3) introducing an engineered Cys^14^ο£ΏCys^39^ bond near the reactive site on the basis of the sequence homology between OMSVP3 and ascidian trypsin inhibitor. This variant exhibits a narrower inhi