𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Assignment of proton NMR resonances and conformational analysis of the K13CK cystatin-like peptide

✍ Scribed by Catherine Samsoen; Evelyne Lebrun; Roland Van Rapenbusch; Daniel Davoust; Gilles Lalmanach

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
282 KB
Volume
30
Category
Article
ISSN
0749-1581

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The assignments of the proton NMR signals and the conformational analysis of the cystatin‐mimicking peptide K13CK (Lys‐Val‐Gly‐Gly‐Gln‐Val‐Val‐Cys‐Gly‐Ala‐Pro‐Trp‐Lys) in aqueous solution at pH 3.2 have been achieved using two‐dimensional DQF‐COSY, HOHAHA and ROESY nuclear magnetic resonance techniques. The chemical shifts, the magnitudes of the coupling constants, the temperature dependences of the amide protons and the intramolecular NOEs were used to obtain information on the conformation. The NMR spectra of cysteine proteinase inhibitor K13CK demonstrate a cis‐trans isomerism at the Ala~10~Pro~11~ peptide bond.

📜 SIMILAR VOLUMES

Assignment of proton NMR spectra and con
Assignment of proton NMR spectra and conformational analysis by two-dimensional heteronuclear relayed correlation NMR spectroscopy
✍ Christopher J. Turner 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 264 KB

The method of two-dimensional heteronuclear relayed correlation spectroscopy was used to establish the assignment of the severely overcrowded part of the proton spectrum of menthol by relating it to the previously assigned carbon spectrum. Extrapolation of the signal-to-noise ratio obtained with ove

Assignment of the 1H and 13C NMR spectra
Assignment of the 1H and 13C NMR spectra and conformational analysis of the pyrrolizidine alkaloid 13-O-acetyldicrotaline
✍ David S. Rycroft; Iain R. Stirling; David J. Robins 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 305 KB

## Abstract The ^1^H and ^13^C NMR spectra of the pyrrolizidine alkaloid 13‐__O__‐acetyldicrotaline were studied at 4.7 T. A full assignment of the ^1^H and ^13^C signals was achieved by application of a variety of NMR techniques, including homonuclear ^1^H NOE difference, 2D δ~H~/δ~H~ phase‐sensit

DNA fragment conformations. I—methods fo
DNA fragment conformations. I—methods for the assignment of DNA fragment proton resonances. 1H NMR spectra of dApTpGpT and dApCpApTpGpT
✍ S. Tran-Dinh; J. M. Neumann; T. Huynh-Dinh; J. Igolen; S. K. Kan 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 404 KB

## Abstract A general method for the assignment of DNA fragment proton resonances, especially for the sugar protons, has been presented and used to interpret the 400 MHz proton spectra of dApTpGpT and dApCpApTpGpT in neutral aqueous solution. Only fine splittings of about 3 Hz are observed in the H

1H and 13C NMR chemical shift assignment
1H and 13C NMR chemical shift assignments and conformational analysis for the two diastereomers of the vitamin K epoxide reductase inhibitor brodifacoum
✍ John R. Cort; Herman Cho 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 134 KB 👁 1 views

## Abstract Proton and ^13^C NMR chemical shifts and ^1^H^1^H scalar couplings for the two diastereomers of the potent vitamin K epoxide reductase (VKOR) inhibitor brodifacoum have been determined at 293 K from acetone solutions containing both diastereomers. To facilitate difficult assignments, h