✦ LIBER ✦
Assignment of 15N-NMR resonances of vitamin B12 analogues by 2D-[15N, 1H] long-range correlation: Fully [15N]-labelled Co-β-cyano-5-hydroxybenzimidazoylcobamide (factor III) and derivatives
✍ Scribed by Roger Hollenstein†; Erhard Stupperich
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- German
- Weight
- 394 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0018-019X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The ^15^N‐NMR spectra of vitamin B~12~ analogues obtained in fully ^15^N‐labelled form have been measured by direct and inverse (^15^N, ^1^H) correlated spectroscopy. All resonances, except those of the NH~2~ groups, have been assigned to individual N‐atoms. The influences on δ (N) are analyzed and discussed which are caused by changing the α‐face ligand from CN to H~2~O or CH~3~ and by switching the β‐face ligand from the base‐on to the base‐off state. An implication of the correct resonance assignment on biosynthetic pathways is demonstrated.