Assigning weight of DNA evidence using a continuous model that takes into account stutter and dropout
✍ Scribed by R. Puch-Solis; T. Clayton; J. Whitaker; S. Pope
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 64 KB
- Volume
- 50
- Category
- Article
- ISSN
- 1355-0306
No coin nor oath required. For personal study only.
✦ Synopsis
achieved full profiles from 70% of samples analysed. Sensitivity experiments were conducted using liquid blood aliquots which were serially diluted to achieve dilutions between 0.1 ng/µl and 0.0007 ng/µl. From eight dilutions, 5 µl aliquots were simultaneously sampled for the automated and manual DNA profiling systems. The results of both systems were assessed using allele scoring as per NDNAD loading criteria. The first sensitivity experiment (n = 3) showed 69% of samples gave equal to or more loadable alleles when profiled using the automated profiling system. The second sensitivity experiment (n=5) showed 39 % of samples gave equal to or more loadable alleles when profiled using the automated profiling system. As the results from the sensitivity experiments are variable we will maintain a manual profiling system for critical samples or samples thought to yield-low concentrations of DNA. Assessing the audit trail for samples showed that on all occasions the sample name was correctly transferred from the extraction batch to the quantification record, PCR record, and DNA electropherogram printout. In conclusion, we have designed a system which can obtain DNA profiles and manage sample data effectively from all the tested sample types such that we can process the majority of samples encountered within SPSA Forensic Services, Glasgow.