Assessment of β-Carotene Content in Sweetpotato Breeding Lines in Relation to Dietary Requirements
✍ Scribed by A.H. Simonne; S.J. Kays; P.E. Koehler; R.R. Eitenmiller
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 497 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0889-1575
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✦ Synopsis
Sweetpotato (Ipomoea batatas, Lam.) (\beta)-carotene represents an important nutritional source of vitamin (A) in a number of areas worldwide. Genetic variation for (\beta)-carotene and the minimum content required among cultivars for different levels of consumption were determined using a cross-section of the sweetpotato germ plasm. (\beta)-Carotene was separated and measured by HPLC using a (V_{y}) dac (\mathrm{C}_{18} 218) TP 54 column and a mobile phase of methanolacetonitrile:tetrahydrofuran (25:28:2). Peak identity was established by spectral comparison using a Shimadzu SPD-M6A photodiode array UV-VIS detector. Recovery was determined by standard addition prior to saponification. Many of the white to pale cultivars did not contain any detectible provitamin A activity ( (\beta)-cryptoxanthin, (\alpha)-carotene, (\beta)-carotene). (\beta)-Carotene levels ranged from 1 to (190 \mu \mathrm{g} \cdot \mathrm{g}^{-1}) dry wt in blanched roots containing detectable levels of (\beta)-carotene. (\beta)-Carotene content was highly correlated with hue angle in lines with yellow to deep orange color ((r=-0.99)) and the relationship can be used for the estimation of (\beta)-carotene. The specific (\beta)-carotene content needed for new cultivars to provide the vitamin A requirement was estimated using the per capita consumption of sweetpotatoes in various geographic areas and the percentage of the RDA to be provided by sweetpotatoes. Populations in many areas of the world would benefit from increased (\beta)-carotene contents in available sweetpotatoes. ici 1993 Academic Press, Inc.