Assessment of viability of hepatocytes in suspension using the MTT assay
โ Scribed by Schiller, C.D.; Kainz, A.; Mynett, K.; Gescher, A.
- Book ID
- 121725112
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 352 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0887-2333
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๐ SIMILAR VOLUMES
Isolated rat hepatocytes are used in many metabolic studies, but the viability of these cell preparations is often not adequately established. The present study shows that ATP content is a more reliable index of metabolic viability than trypan blue exclusion. At some of the low trypan blue exclusion
The new 3-(4,5-dimethylthiazol-2yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5[(phenylamino)carbonyl]-2Htetrazolium hydroxide (XTT) calorimetric assays for assessing hepatocyte density, viability and proliferation were