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Assessment of the prevalence of the 985A>G MCAD mutation in the French-Canadian population using allele-specific PCR

✍ Scribed by S Giroux; A Dubé-Linteau; G Cardinal; Y Labelle; N Laflamme; Y Giguère; F Rousseau


Book ID
110888404
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
235 KB
Volume
71
Category
Article
ISSN
0009-9163

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We have developed an improved allele-specific polymerase chain reaction (AS-PCR) procedure that can selectively amplify mutant DNA sequences (which differ from the normal sequences by a single base pair) in the presence of large excess of normal sequences. We applied this procedure to quantification