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Assessment of the efficiency of cryophylactic agents in a cell-free system

โœ Scribed by J.W. Winkert; W.N. Fishbein


Book ID
115849036
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
231 KB
Volume
13
Category
Article
ISSN
0011-2240

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Two modifications are introduced to convert the Escherichia coli cell-free extract ("S30") into a high efficiency system for coupled transcription/translation of exogenously added genes. (a) The ribosome fraction collected from the S30 by ultracentrifugation is used. It contains all the proteins nec