Assessment of substrates for horseradish peroxidase in enzyme immunoassay
β Scribed by Elham Al-Kaissi; Ann Mostratos
- Book ID
- 118899081
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 270 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0022-1759
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π SIMILAR VOLUMES
Horseradish peroxidase (HRP) catalyzes the oxidative chemiluminescent reaction of luminol, and firefly luciferase catalyzes the oxidation of firefly D-luciferin. Here we report a novel substrate, 5-(5-azoluciferinyl)-2,3-dihydro-1,4-phthalazinedione (ALPDO), that can trigger the activity of HRP and
## 306 (12) and 3.2 times greater than the very sensitive method Comparison of Three Chemiluminescent of Saheki et al. (13), in which the phosphomolybdate com-Horseradish Peroxidase Substrates for plex is reduced by ascorbic acid. At this point, it must be Immunoblotting emphasized that the ascorb