Assessment of radiation-induced DNA strand breaks and their repair in unlabeled cells
โ Scribed by Maja Item Affentranger; Werner Burkart
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 636 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
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A widely accepted assumption in radiobiology is that ionizing radiation kills cells by inducing forms of damage in DNA structures that lead to the formation of lethal chromosome aberrations. One goal of radiation biology research is the identification of these forms of DNA damage, the characterizati
The alkaline/filter DNA elution technique measures single-strand DNA breaks in mammalian cells based on the DNA molecular weight dependent retention of the macromolecule on 2-@m-pore-size filters. Described here is a modification of the technique which uses [rH]thymidine-labeled DNA of y-irradiated