Assessment of proliferative activity in carcinomas of the human alimentary tract by Ki-67 immunostaining

✍ Scribed by Rainer Porschen; Anja Kriegel; Christa Langen; Susanne Classen; Martina Hilse; Bernd Lohe; Klaus-Jürgen Hengels; Franz Borchard

• Book ID: 102867580
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 737 KB
• Volume: 47
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910470510

✦ Synopsis

The monoclonal antibody (MAb) Ki-67, which is directed against a proliferation-associated nuclear antigen, was used to measure tumor proliferation in 165 carcinomas of the esophagus, stomach, colon and rectum with an indirect immunoperoxidase technique. The percentage of Ki-67-positive tumor cells (Ki-67 index) was evaluated with the point-counting method. The Ki-67 index in gastric cancers (mean, 24.8%; standard deviation, I I. I %) was significantly lower than in tumors of the esophagus 35.7 + 12.6%), colon (37.6 -C 15.2%), (5.9-75.3%) could be observed within the various tumor types. In recurrent colorectal carcinomas, the Ki-67 index significantly increased to 51.9%. The Ki-67 index was independent of pathologic (e.g., TNM-stage, grading, tumor volume, tumor site) and clinical variables (age and gender of the patients). A marked heterogeneity of Ki-67 expression within different tumor stages was noted. Statistically significant regional variations in tumor proliferation existed between different areas within the same tumor. and rectum (34.3 f 16.4X). 6 -A wide range of the Ki-67 index 3To whom correspondence and reprint requests should be sent.