Abstract
This study examined the feasibility of using short‐echo water‐suppressed point‐resolved spectroscopy (PRESS) on a clinical 3T magnetic resonance (MR) scanner for evaluating biochemical changes in degenerated bovine and cadaveric human intervertebral discs. In bovine discs (N = 17), degeneration was induced with papain injections. Degeneration of human cadaveric discs (N = 27) was assessed using the Pfirrmann grading on T~2~‐weighted images. Chemicals in the carbohydrate region (Carb), the choline head group (Cho), the N‐acetyl region (N‐acetyl), and the lipid and lactate region (Lac+Lip) were quantified using ^1^H PRESS, and were compared between specimens with different degrees of degeneration. The correlation between the spectroscopic findings and glycosaminoglycan (GAG) quantification using biochemical assays was determined. Significant differences were found between the ratios (N‐acetyl/Cho, N‐acetyl/Lac+Lip) acquired before and after papain injection in bovine discs. For human cadaveric discs, significant differences in the ratios (N‐acetyl/Carb, N‐acetyl/Lac+Lip) were found between discs having high and low Pfirrmann scores. Significant correlations were found between N‐acetyl/Lac+Lip and GAG content in bovine discs (R = 0.77, P = 0.0007) and cadaveric discs (R = 0.83, P < 0.0001). Significant correlation between N‐acetyl/Cho and GAG content was also found in cadaver discs (R = 0.64, P = 0.0039). This study demonstrates for the first time that short‐echo PRESS on a clinical 3T MR scanner can be used to noninvasively and can reproducibly quantify metabolic changes associated with degeneration of intervertebral discs. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.