Assessment of EGFR and TGF-alpha expression in relationship to HPV status and KI-67 distribution in cervical intraepithelial neoplasms
✍ Scribed by Athanassios Dellas; Elisabeth Schultheiss; Alfonso C. Almendral; Joachim Torhorst; Fred Gudat
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 551 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Expression of epidermal-growth-factor receptor (EGFR), transforming growth factor alpha (TGF-a) and Ki-67 proliferation antigen in cervical intra-epithelial neoplasms were analyzed. To examine the interrelationship of TGF-a, EGFR, Ki-67 and HPV status in dysplasia and carcinoma in situ, formalin-fixed tissue sections of 92 women were immunostained with monoclonal antibodies to EGFR, TGF-a and Ki-67. The presence of HPV was assessed by in situ DNA hybridization. The highest positive TGF-a expression was seen in the group of mild dysplasia. The difference was significant between the relatively high expression in mild dysplasia and the low occurrence in severe dysplasia and carcinoma in situ as well. The same relation could be found between TGF-a expression in papilloma-virus-negative dysplasia and those with the presence of HPV 16/ 18. In contrast to these findings, the Ki-67 proliferation marker was intensely detectable in severe dysplasia and carcinoma in situ. Ki-67stained neoplastic cell n d e i were found in a significantly higher percentage of HPV-positive than in HPV-negative lesions. TGF-a over-expression is obviously combined with low proliferating activity and vice versa. Irrespective of the grade of dysplasia or HPV status, EGFR was expressed abnormally as compared with normal squamous epithelium. Over-expression of TGF-a in mild dysplasia could be associated with the autocrine pathway of cell-growth regulation. In the presence of HPV 16/ 18 the EGFR/TGF-a pathway for growth stimulation is probably not involved.