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Assessment of DNA damage in individual hamster embryos by comet assay

✍ Scribed by Masashi Takahashi; Norie Saka; Hitomi Takahashi; Yukio Kanai; Richard M. Schultz; Akira Okano


Book ID
111659332
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
248 KB
Volume
54
Category
Article
ISSN
1040-452X

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Assessment of DNA damage in filamentous
✍ Alexander Hahn; Bertold Hock πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 64 KB

The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph