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Assessment of Candida shehatae viability by flow cytometry and fluorescent probes

✍ Scribed by Monthéard, Julie; Garcier, Sandy; Lombard, Eric; Cameleyre, Xavier; Guillouet, Stéphane; Molina-Jouve, Carole; Alfenore, Sandrine

Book ID
119326838
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
570 KB
Volume
91
Category
Article
ISSN
0167-7012

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✍ J. P. Diaper; K. Tither; C. Edwards 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 441 KB

The ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (Rh123); 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] and fluorescein diacetate (FDA). Rh123 was found to clearly differentiate viable from non-viable bacteria. The methodo