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Assessing the permissiveness of transcriptional activator binding sites

✍ Scribed by Steven P. Rowe; Anna K. Mapp

Publisher: Wiley (John Wiley & Sons)
Year: 2008
Tongue: English
Weight: 231 KB
Volume: 89
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.20946

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✦ Synopsis

Abstract

Both genetic and biochemical data suggest that transcriptional activators with little sequence homology nevertheless function through interaction with a shared group of coactivators. Here we show that a series of peptidomimetic transcriptional activation domains interact under cell‐fiee and cellular conditions with the metazoan coactivator CBP despite differences in the positioning and identity of the constituent functional groups. Taken together, these results suggest that a key activator binding site within CBP is permissive, accepting multiple arrangements of hydrophobic functional groups. Further, this permissiveness is also observed with a coactivator from S. cerevisiae. Thus, the design of small molecule mimics of transcriptional activation domains with broad function may be more straightforward than previously envisioned. © 2008 Wiley Periodicals, Inc. Biopolymers 89: 578–581, 2008.

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]

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