Assessing hydrophobic regions of the plasma membrane H+-ATPase from Saccharomyces cerevisiae
β Scribed by Donna Seto-Young; Brian C. Monk; David S. Perlin
- Book ID
- 113268962
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 632 KB
- Volume
- 1102
- Category
- Article
- ISSN
- 0167-4838
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
1. Plasma membrane preparations have been isolated from spheroplasts of Saccharomyces cerevisiae, strain R XII, via lysis and subsequent differential centrifugation. These preparations are almost devoid of mitochondrial contamination. 2. The plasma membrane ATPase is fairly stable when refrigerated,
1. The distribution of ATPase and several marker enzymes was examined after differential and sucrose gradient centrifugation of yeast homogenates. 2. An ATPase activity not sensitive to oligomycin is found exclusively associated with a particulate fraction equilibrating at densities of 1.23-1.25. Th