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Assembly of the Sea Urchin Fertilization Membrane: Isolation of Proteoliaisin, a Calcium-Dependent Ovoperoxidase Binding Protein

✍ Scribed by Peggy J. Weidman, Erica S. Kay and Bennett M. Shapiro


Book ID
124787708
Publisher
Rockefeller University Press.
Year
1985
Tongue
English
Weight
927 KB
Volume
100
Category
Article
ISSN
0021-9525

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We have purified and characterized a collagenase/gelatinase activity expressed during sea urchin embryonic development. The native molecular mass was determined to be 160 kDa, while gelatin substrate gel zymography revealed an active species of 41 kDa, suggesting that the native enzyme is a tetramer