Assembly of Anionic Conjugated Polymer with 6-O-Modified PNP-β-Galactoside for Fluorescence Logic-signal-based Multiplex Detections of Enzymes

✍ Scribed by Ya-juan Wang; Bao-juan Xin; Xin-rui Duan; Guo-wen Xing; Shu Wang

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 359 KB
• Volume: 31
• Category: Article
• ISSN: 1022-1336
• DOI: 10.1002/marc.201000165

✦ Synopsis

Abstract

Anionic conjugated polymer (PFP‐SO) was assembled with a novel enzymatic substrate 6‐O‐modified PNP‐β‐galactoside (1) for sensitive multiplex enzyme detections. The PFP‐SO/1/lipase/β‐galactosidase system has two chemical input signals which are Input 1 (lipase) and Input 2 (β‐galactosidase), and output optical signals such as fluorescence emission at 416 nm or 450 nm. Four types of logic gates, including YES, INH, NAND and AND, were successfully constructed and utilized for multiplex detections of lipase and β‐galactosidase in one tube.

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