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Assembly and Degradation of Low-Fouling Click-Functionalized Poly(ethylene glycol)-Based Multilayer Films and Capsules

✍ Scribed by Melissa K. M. Leung; Georgina K. Such; Angus P. R. Johnston; Dhee P. Biswas; Zhiyuan Zhu; Yan Yan; Jean-François Lutz; Frank Caruso


Book ID
104593696
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
770 KB
Volume
7
Category
Article
ISSN
1613-6810

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✦ Synopsis


Abstract

Nano‐/micrometer‐scaled films and capsules made of low‐fouling materials such as poly(ethylene glycol) (PEG) are of interest for drug delivery and tissue engineering applications. Herein, the assembly and degradation of low‐fouling, alkyne‐functionalized PEG (PEG~Alk~) multilayer films and capsules, which are prepared by combining layer‐by‐layer (LbL) assembly and click chemistry, are reported. A nonlinear, temperature‐responsive PEG~Alk~ is synthesized, and is then used to form hydrogen‐bonded multilayers with poly(methacrylic acid) (PMA) at pH 5. The thermoresponsive behavior of PEG~Alk~ is exploited to tailor film buildup by adjusting the assembly conditions. Using alkyne–azide click chemistry, PEG~Alk~/PMA multilayers are crosslinked with a bisazide linker that contains a disulfide bond, rendering these films and capsules redox‐responsive. At pH 7, by disrupting the hydrogen bonding between the polymers, PEG~Alk~ LbL films and PEG~Alk~‐based capsules are obtained. These films exhibit specific deconstruction properties under simulated intracellular reducing conditions, but remain stable at physiological pH, suggesting potential applications in controlled drug release. The low‐fouling properties of the PEG films are confirmed by incubation with human serum and a blood clot. Additionally, these capsules showed negligible toxicity to human cells.