Assay validation and characterization of hepatic 5′-deiodinase activity in ring doves using reverse-T3 as substrate

Hepatic 5′-deiodinase activity of Japane

Hepatic 5′-deiodinase activity of Japanese quail using reverse-T3 as substrate: Assay validation, characterization, and developmental studies

✍ Freeman, Thomas B. ;McNabb, F. M. Anne 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 954 KB

## Abstract Using rT3 as substrate, an in vitro 5′D assay was validated for use with liver tissue from adult Japanese quail, by defining conditions under which activity is proportional to enzyme (protein) concentration and is linear with incubation time. Activity was measured as the release of ^125