Assay of β-galactosidase activity by Flow Injection Analysis (FIA)

✍ Scribed by J. Cairó; M. Vidal; A. Villaverde; F. Valero; F. J. Lafuente; C. Solà

• Book ID: 104655062
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 236 KB
• Volume: 5
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00185020

✦ Synopsis

A novel method to analyze O-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL. being the detection limit 25 UjmL with 55 samples per hour frecuency and a RSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia. co/i cultures producing 8-galactosidase.