𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Assay of the Concentration and 13C Enrichment of Acetate and Acetyl-CoA by Gas Chromatography-Mass Spectrometry

✍ Scribed by F. David; M. Beylot; M.W. Reider; V.E. Anderson; H. Brunengraber

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
460 KB
Volume
218
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

We present two techniques for determining the concentration and ({ }^{13} \mathrm{C}) enrichment of acetate in biological fluids. After the sample has been spiked with an internal standard of (\left[2,2,2,{ }^{2} \mathrm{H}_{3}, 1-{ }^{13} \mathrm{C}\right]) acetate, acetate is first enzymatically converted to acetyl-coenzyme (A), which is chemically converted to acetylglycine. The latter is analyzed by gas chromatography-mass spectrometry, either as a methyl ester by positive chemical ionization or as a pentafluorobenzyl ester by negative chemical ionization. The mole percentage enrichment of tissue acetyl-CoA can also be assayed after conversion to acetylglycine pentafluorobenzyl ester. O 1994 Academic Press, Inc.

📜 SIMILAR VOLUMES

Assay of the Concentration and 13C Isoto
Assay of the Concentration and 13C Isotopic Enrichment of Propionyl-CoA, Methylmalonyl-CoA, and Succinyl-CoA by Gas Chromatography–Mass Spectrometry
✍ Takhar Kasumov; Wenjun Z. Martini; Aneta E. Reszko; Fang Bian; Bradley A. Pierce 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 85 KB

We developed gas chromatography-mass spectrometry assays for the concentration and mass isotopomer distribution of propionyl-CoA, methylmalonyl-CoA, and succinyl-CoA in tissues. The assays involves perchloric acid extraction of the tissue, spiking the extract with [ 2 H 5 ]propionyl-CoA and [ 2 H 4

Assay of the Acetyl-CoA Probe Acetyl-Sul
Assay of the Acetyl-CoA Probe Acetyl-Sulfamethoxazole and of Sulfamethoxazole by Gas Chromatography-Mass Spectrometry
✍ Z. Ying; K.C. Agarwal; M. Beylot; M.V. Soloviev; F. David; M. Reider; K.Y. Tsern 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 441 KB

We present gas chromatographic-mass spectrometric assays for (i) the concentration of sulfamethoxazole and (ii) the concentration and molar percentage enrichment of acetyl-sulfamethoxazole in biological fluids. The compounds are extracted with ethyl acetate, derivatized with either diazomethane or p

Assay of the Concentration and 13C-Isoto
Assay of the Concentration and 13C-Isotopic Enrichment of Malonyl–Coenzyme A by Gas Chromatography–Mass Spectrometry
✍ Aneta E. Reszko; Takhar Kasumov; Blandine Comte; Bradley A. Pierce; France David 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 90 KB

We developed gas chromatography-mass spectrometry assays for the concentration and mass isotopomer distribution of malonyl-CoA in tissues. The assay involves perchloric acid extraction of the tissue, spiking the extract with [U-13 C 3 ]malonyl-CoA or dimethylmalonyl-CoA internal standard, isolation

Determination of (13C)urea enrichment by
Determination of (13C)urea enrichment by gas chromatography/mass spectrometry and gas chromatography/isotope ratio mass spectrometry
✍ Michel Beylot; France David; Yadh Khalfallah; Sylvie Normand; Valerie Large; Hen 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 354 KB 👁 2 views

We present gas chromatographic/mass spectrometric and gas chromatographic/isotope ratio mass spectrometric assays of the 13C enrichment of plasma urea converted to its dimethylaminomethylene derivative. The limits of sensitivity of the two techniques are 0.2% and 0.02%, respectively. The techniques

Assay of the13C and2H Mass Isotopomer Di
Assay of the13C and2H Mass Isotopomer Distribution of Phosphoenolpyruvate by Gas Chromatography/Mass Spectrometry
✍ Previs, Stephen F.; Rosiers, Christine Des; Beylot, Michel; David, France; Brune 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 582 KB

The 13C mass isotopomer distribution of liver phosphoenolpyruvate (PEP) yields important information on the regulation of gluconeogenesis and the citric acid cycle. A convenient technique is presented for measuring the mass isotopomer distribution of PEP in tissue extracts. The procedure involves re