Assay of salicylic acid and related compounds in plant cell cultures by capillary GC
✍ Scribed by R. A. Budi Muljono; A. M. G. Looman; R. Verpoorte; J. J. C. Scheffer
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 57 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
A capillary gas chromatographic (GC) method for the assay of salicylic acid (1) and related compounds in plant cell cultures is described. The gas chromatograms were validated qualitatively by means of GC-mass spectrometry. The detection limits of 1 (50 ng) and 2,3-dihydroxybenzoic acid (2; 125 ng), obtained in the GC split injection mode, could be lowered 25-to 50-fold by using the splitless mode. Derivatization of these compounds with diazomethane gave the methyl esters as major derivatization products in yields of 98% and 94%, respectively. The extraction recoveries of 1 and 2 from lyophilized Catharanthus roseus cells were more than 95%, using o-anisic acid as internal standard, and tetrahydrofuran was found to be a suitable extraction solvent.