Assay of Pancreatic Lipase with the Surface Acoustic Wave Sensor System

✍ Scribed by K. Ge; D.H. Liu; K. Chen; L.H. Nie; S.Z. Yao

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 339 KB
• Volume: 226
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1215

✦ Synopsis

A new type of surface acoustic wave sensor system for assaying the activity of pancreatic lipase has been proposed. The assay of this enzyme is based on the change in conductance of the solution caused by the release of a fatty acid, using triolein as a substrate. A linear relationship between frequency response and enzyme concentration is obtained. Kinetic parameters of pancreatic lipase, i.e., the Michaelis constant for triolein and the corresponding maximum initial rate, are estimated to be (5.46 \mathrm{~mm}) and (7551.6 \mathrm{~Hz} / \mathrm{min}), respectively. The effects of temperature and (\mathrm{pH}) value are also investigated. (c) 1995 Academic Press, Inc.