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Assay of l-3-hydroxyacyl-coenzyme A dehydrogenase with substrates of different chain lengths

✍ Scribed by Xue-Ying He; Song-Yu Yang; Horst Schulz

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 593 KB
Volume: 180
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(89)90095-x

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✦ Synopsis

A method for assaying L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) which permits rate measurements with t-3-hydroxyacyl-CoA substrates of various chain lengths at physiological pH is described. The method is based on a coupled assay system in which 3ketoacyl-CoAcompounds formed by the dehydrogenase are cleaved by 3-ketoacyl-CoA thiolase (EC 2.3.1.16) in the presence of CoASH. The advantages of this assay method are its irreversibility and elimination of product inhibition. The assay procedure was used to determine the kinetic parameters (K,, V,,,,,) of pig heart L-3-hydroxyacyl-CoA dehydrogenase with several substrates of various chain lengths. The data obtained show the enzyme to be most active with medium-chain substrates whereas K,,, values for medium-chain and long-chain substrates are almost equal but much lower than those previously reported. 8 1~8s Academic press. lnc.

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