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Assay of intracellular thymidylate synthetase activity and inhibition by 5-fluoro-2′-deoxyuridine in lymphocytes

✍ Scribed by Susan Cox; Johan Harmenberg


Book ID
116098134
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
279 KB
Volume
25
Category
Article
ISSN
0165-022X

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The inhibitors of DNA synthesis, 5-fluoro-2'-deoxyuridine and hydroxyurea, caused an inhibition of thymidine kinase, replicative DNA polymerase and CDP reductase activities in stimulated lymphocytes when they were exposed to the inhibitors during the early transformation period (0-17 hr). However, t